Fig. 3.

Total ACC activity is increased by MIG12. ACC was partially purified from livers of mice and MIG12 was produced as described in Methods. (A) Partially purified ACC (55 μg) was preincubated with the indicated amounts of MIG12 or BSA, and 1.0 mM citrate for 30 min and ACC activity assays were performed as described in Methods. Each value is the mean ± S.E. of three independent reactions. Similar results were obtained in three independent studies. (B) Partially purified ACC (55 μg) was preincubated with 20 μg of recombinant MIG12 or BSA and ACC activity was measured in the presence of increasing concentrations of citrate as described in Methods. Each value is the mean ± S.E. of three independent reactions. Similar results were obtained in three independent studies.