Fig. 5.

Validation of selected target proteins labeled by sulfoxythiocarbamate analog 8f in cells. (A) Target proteins labeled by 8f. HEK293 cells were treated with 8f or vehicle for 2.5 h. (B) Competition by sulforaphane or TP225. HEK293 cells were pretreated with sulforaphane, TP225, or vehicle for 0.5 h before incubation with 8f for 0.5 h. Each protein was immunoprecipitated from cell lysates, subjected to click reaction with biotin azide on beads. Eluted samples were immunoblotted with streptavidin as well as protein specific antibodies.