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. 2010 May 10;107(21):9831–9836. doi: 10.1073/pnas.1002551107

Fig. 2.

Fig. 2.

SdiA regulation of the gad acid-resistance system. (A) qRT-PCR of the gadW gene encoding the master regulator of the gad system in WT EHEC (86-24), sdiA mutant, sdiA mutant with pACYC177, and sdiA mutant complemented with pDH6 (sdiA in pACYC177) strains grown in DMEM to an OD600 of 1.0 in the absence and presence of AHL (10 μM oxo-C8-HSL and 10 nM oxo-C8-HSL). (B) qRT-PCR of the gadX gene in WT EHEC (86-24), sdiA mutant, sdiA mutant with pACYC177, and sdiA mutant complemented with pDH6 (sdiA in pACYC177) strains grown in DMEM to an OD600 of 1.0 in the absence and presence of AHL (10 μM oxo-C8-HSL). (C) Acid resistance (survival in acidic pH) through the gad system in the absence and presence of 10 μM oxo-C8-HSL. In conditions where no AHLs were added, the same amount of the ethyl-acetate solvent was added to ensure that the solvent had no effect in gene expression.