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. 2010 May 10;107(21):9677–9682. doi: 10.1073/pnas.1004431107

Fig. 1.

Fig. 1.

Targeted gene expression. (A) Targeted RNAi knockdown of Mgat1 in the wild-type CNS affects Drosophila life span. The conditional ELAV-GeneSwitch line was used to knock down Mgat1 in the CNS of wild-type flies. Transgene expression from ELAV-GeneSwitch was induced in the presence of the drug RU486 to down-regulate an Mgat1 RNAi line (no. 13) and life span was measured as previously described (5). The complete genotype and life span of the line are indicated in Table 1. (B) Targeted expression of Mgat1 in the CNS of Mgat11 null Drosophila affects life span. Mgat11 null mutants have a maximum life span of 15 days whereas wild-type flies live to 93 days. Targeted expression of Mgat1 to the CNS of Mgat11 null mutants [Mgat1 (+RU)] increases the mean and maximum life spans by 73 and 77%, respectively, relative to wild-type flies and by 135% relative to Mgat11 null mutant flies that were not treated by drug [Mgat1 (−RU)]. All life span plots were derived from a single population of flies.