Fig. 2.

Life span studies on Mgat1 rescued Mgat1¹ null flies. (A) The effect of oxidative stress on the life span of Mgat1 rescued flies. Resistance to oxidative stress was measured in adult Mgat1¹ null mutant flies that expressed a wild-type Mgat1 transgene in the CNS [Mgat1 (+RU)] compared with genetically matched controls that did not express Mgat1 [Mgat1 (−RU)]. Briefly, flies were fed normal food containing 3% hydrogen peroxide and their life span was measured over a 180-h period. The Mgat1 rescued flies [Mgat1 (+RU)] lived significantly longer under conditions of oxidative stress (mean life span = 127.9 ± 2.4 h) than control flies (mean life span = 80.8 ± 1.7 h). (B) The effect of percentage of yeast in the diet on the life span of Mgat1 rescued flies. The effect of dietary restriction on the life span of Mgat1 rescued flies [Mgat1 (+RU)] compared with Mgat1¹ null mutant flies that were not treated by drug [Mgat1 (−RU)] and to wild-type controls was determined by varying the amount of dietary yeast from 2 to 8% as described (34). Shown is mean life span with SE bars. Asterisks above the line for Mgat1 rescued flies [Mgat1 (+RU)] indicate that survival differed significantly between the Mgat1 rescued flies and controls that were not treated by drug [Mgat1 (−RU)] at yeast concentrations from 2 to 8% using the log-rank rest (P < 0.001). Asterisks between the lines for controls that were not treated by drug [Mgat1 (−RU)] and wild-type flies indicate that survival differed significantly between Mgat1 rescued flies [Mgat1 (+RU)] and wild-type flies only at 2% yeast, using the log-rank rest (P < 0.001). All life span plots were derived from a single population of flies. The standard media used for the data shown in Table 1 contained 2% yeast.