Table 2.

Hepatoprotective activity of the total alkaloid fraction of Hygrophila auriculata leaves on CCl₄ intoxicated HepG2 cells

Treatment	Concentration (µg/ml)	% Viability
Control	-	100
CCl4	-	17.65 ± 2.16a
CCl4 (1%) + standard silymarin	250	90.14 ± 3.12b
CCl4 (1%) + total alkaloid fraction	80	92.52 ± 4.21b
	70	90.15 ± 3.94b
	60	85.32 ± 3.65b
	50	80.43 ± 4.01b
	40	78.05 ± 3.03b

Average of 5 determinations (n=5), 4 replicates;

^a= P < 0.001, when compared to the normal cells;

^b= P < 0.01, when compared to the CCl₄ intoxicated cells.