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. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 10;396(4):921–926. doi: 10.1016/j.bbrc.2010.05.023

Figure 2. The PPARγ agonist troglitazone induces βarr1/2 recruitment to the AT1AR in a Gq protein-independent manner.

Figure 2

(A) FRET analysis was used to assess the Gq protein-mediated response to agonist stimulation by detecting changes in the FRET ratio of transiently-transfected DAGR in HA-AT1AR cells. Ang II (100nM) stimulation induced a rapid increase in DAGR ratio which peaked at 90 sec, while Trog (100μM) stimulation produced no response in DAGR ratio, n = 3 independent experiments. (B) HA-AT1AR cells were stimulated for 5 min with Ang II (1–10nM) or Trog (10–100μM) and underwent crosslink/IP with HA-agarose gel. βarr1/2 was recruited to HA-AT1AR in a concentration-dependent manner in response to both Ang II and Trog, as summarized in histogram. *P<0.05, †P<0.01 and ‡ P<0.001 versus non-stimulated cells (NS), n = 6 each.