(A) HA-AT1AR cells were stimulated with Ang II (5nM) ± Losartan (Los, 10μM) or Trog (50μM) ± Los (100μM) for 5 min and underwent crosslink/IP as described in Figure 2B. βarr1/2 recruitment to AT1AR was significantly increased following stimulation with either Ang II or Trog, an effect blocked by 5 min Los pretreatment, as summarized in histogram. †P<0.01 and ‡ P<0.001, n ≥ 3 each. (B) FLAG-β1AR cells were stimulated with ISO (1μM) or Trog (100μM) for 5 min and underwent crosslink/IP with FLAG-M2 agarose. βarr1/2 recruitment to β1AR was significantly increased following stimulation with ISO, but not Trog, as summarized in histogram. ‡ P<0.001 versus cells with no pharmacological treatment, n = 3 each.