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. 2009 Dec 2;29(48):15134–15144. doi: 10.1523/JNEUROSCI.4720-09.2009

Figure 2.

Figure 2.

Acetylation of full-length ataxin-7. A, Myc-tagged full-length ataxin-7-10Q, ataxin-7-92Q, and empty vector were expressed in 293T cells. Immunoprecipitation followed by immunoblot analysis were carried out using cell lysates transfected with ataxin-7. Blots were probed with monoclonal acetyl lysine antibody (lower panel). The arrowheads represent ataxin-7-10Q or ataxin-7-92Q full-length and fragments acetylated. B, MoPrP-flag-SCA7-10Q-myc and MoPrP-flag-SCA7-92Q-myc transgenic cerebellar mouse lysates were immunoprecipitated with anti-ataxin-7. Western blots of the immunoprecipitated lysates were probed with anti-ataxin-7 and with monoclonal anti-acetyl lysine antibody.