Figure 5.
MUC-CD targeted 4H11-28z+ T cells traffic to peritoneal OV-CAR3(MUC-CD/GFP-FFLuc) tumors following systemic intravenous infusion resulting in efficient anti-tumor efficacy. (A) BLI of tumor progression of representative i.p. and i.v. 4H11-28z+ T cell treated mice with ultimately progressive disease following treatment compared to BLI of tumor progression in a representative control 19-28z+ T cell treated mouse. (B) Kaplan-Meier survival curve of SCID-Beige mice treated i.p. or i.v. with 4H11-28z+ T cells. Tumor eradication is enhanced after either ip or iv infusion of 4H11-28z+ T cells when compared to control treated mice. Both ip and iv 4H11-28z+ T cell treated mice exhibited statistically enhanced survival when compared to 19-28z+ T cell treated control cohorts while survival between the i.p. and i.v. treated 4H11-28z+ T cell cohorts was not statistically significant (p=0.22). (C) Systemically injected CFSE stained 4H11-28z+ T cells traffic to advanced i.p. OV-CAR(MUC-CD) tumors. Presence of i.v. injected CFSE labeled 19-28z+ control T cells (left panel) and 4H11-28z+ T cells (right panel) 1 day following infusion into SCID-Beige mice with OV-CAR(MUC-CD) tumors injected 7 days earlier as assessed by FACS analysis of single cell OV-CAR3(MUC-CD) tumor suspensions.