TABLE 1.
Comparison of experimental design between the crosses
| Experimental design | Cross I | Cross II | |
|---|---|---|---|
| Number of mice | |||
| Tissue | Sex | ||
| Adipose | Female | 147 | 136 |
| Male | 143 | 133 | |
| Brain | Female | 133 | 140 |
| Male | 118 | 149 | |
| Liver | Female | 149 | 141 |
| Male | 142 | 156 | |
| Muscle | Female | 158 | 123 |
| Male | 139 | 148 | |
| Diets | |||
| Purina Chow containing 4% fat (Ralston Purina, St. Louis) | 8 wk | 8 wk | |
| Western diet 88137 containing 42% fat and 0.15% cholesterol (Harlan, Teklad, Madison, WI) | 16 wk | 12 wk | |
| Duration of fasting | 4 hr | 12 hr | |
| Age of mice when killed | 24 wk | 20 wk | |
| Genotyping platform/density of SNPs | ParAllele 1100 SNP array ± 300 SNPs | ParAllele 2000 SNP array (n = 150); Illumina 1536 SNP array (n = 150) | |
| Number of SNPs in study | 1,326 | 1,326 | |
| Expression array platforms | Agilent Mouse TOE75K Array1 | Agilent Mouse TOE75K Array1 | |
| Number of transcripts | |||
| Adipose | 23,568 | 23,560 | |
| Brain | 23,568 | 23,563 | |
| Liver | 23,568 | 23,558 | |
| Muscle | 23,458 | 23,458 | |