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Cyclin D1 regulates sex steroid converting enzymes in HuH7 cells. Cells were cultured in the presence of 10% charcoal-stripped FBS. Cells were treated with cyclin D1 or control small interfering RNA (siRNA) 2 days prior to harvest and cultured with 10 nM DHEA for the final 24 h as indicated. A: Western blot analysis (top) and RT-PCR (bottom) of cyclin D1 expression. B: RT-PCR for the indicated genes from cells cultured in the presence and absence of DHEA as described above.
