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Electron microscopic immunocytochemistry of the kidney in freshwater-acclimated eel, incubated with anti-Na+/K+-ATPase (A–C) and the preimmune serum as a control (D). The immunoreaction was seen on the basal infoldings of the cells composing the first (A) and second (B) segments of the proximal tubule and the distal tubule (C), but absent in the control (a distal tubule cell) incubated with preimmune serum (D). bi, basal infolding; m, mitochondrion; n, nuclei. Bar = 500 nm.
