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Confocal laser scanning micrographs of thick kidney slices (200-μm) of freshwater-acclimated eel, immunostained with anti-Na+/K+-ATPase (red, A) and double-stained with anti-Na+/K+-ATPase and wheat germ agglutinin (WGA) (green, B). Inset, magnified view of a WGA-positive glomerulus (G). PT, proximal tubule; DT, distal tubule; CD, collecting duct. Bar = 50 μm.
