FIG. 5.

Reduced concentrations of LNA result in a decrease in the amount of caspase 3 cleavage. Quantitative RT-PCR of mature Mir21 in cultured granulosa cells with 1.25, 5, or 20 nM LNA-NS or LNA-21 (A). Cells transfected with 1.25 nM LNA-21 had comparable fold reductions in mature Mir21 to those transfected with 42 nM anti-21. Data (n = 3) were normalized to Gapdh; two-way ANOVA was used to determine statistical significance. Means ± SEM with different letters above the bar are different (P < 0.05). B) Representative Western blot (n = 3) of caspase 3, cleaved (active) caspase 3, and ACTB in granulosa cells transfected with 1.25, 5, or 20 nM LNA-NS or LNA-21.