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Real Time PCR Graphs for Chosen Genes in Ishikawa H cells. Data from qPCR confirmed the change in expression from the arrays for genes DUSP6 (dual specificity phosphatase 6), EGR1 (early growth response 1), GRLF1 (glucocorticoid receptor DNA binding factor), and SPHK2 (sphingosine kinase 2) in response to EGF and gefitinib at 12 and 24 h. Calibration was to the corresponding control at the specific time points.
