Table 1.
Primer Sets for Amplification of EGFR Isoforms and Mutants.
| Primer set name | Sequence | Expected band size (bp) |
|---|---|---|
| EGFR forward extracellular 1 (Xcell 1) | ccagtattgatcgggagagc | 1873 |
| EGFR reverse extracellular 1 (Xcell 1) | acaacaccctggtctggaag | |
| EGFR forward extracellular 2 (Xcell 2) | agcctccagaggatgttcaa | 1318 |
| EGFR reverse extracellular 2 (Xcell 2) | tggttttctgaccggaggt | |
| EGFR forward extracellular 3 (Xcell 3) | caaaaactgcacctccatca | 854 |
| EGFR reverse extracellular 3 (Xcell 3) | ggatcttaggccccattcgtt | |
| EGFR forward intracellular (Intracell) | cctaagatcccgtccatcg | 1769 |
| EGFR reverse intracellular (Intracell) | ttggtcctgggtatcgaaag | |
| EGFR isoform B forward | aacaacaccctggtctggaa | 160 |
| EGFR isoform B reverse | tgaagcaaagggagaaattga | |
| EGFR isoform C forward | ggatattctgaaaaccgtaaaggaaa | 96 |
| EGFR isoform C reverse | cgaaaagttctctctaaaacactgatt | |
| EGFR isoform D forward | ccagtgtgcccactacattg | 221 |
| EGFR isoform D reverse | cgctgccatcattactttga | |
| Sequencing primers (exon 18-22) forward | ccaaccaagctctcttgagg | 540 |
| Sequencing primers (exon 18-22) reverse | tgataggcactttgcctcct | |