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. 1961 Feb;40(2):423–432. doi: 10.1172/JCI104270

THE INCORPORATION OF RADIOACTIVE PHOSPHORUS INTO THE PHOSPHOLIPIDS OF HUMAN LEUKEMIC LEUKOCYTES AND PLATELETS*

Barry G Firkin 1,, William J Williams 1,
PMCID: PMC290736  PMID: 13699761

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. BRECHER G., CRONKITE E. P. Morphology and enumeration of human blood platelets. J Appl Physiol. 1950 Dec;3(6):365–377. doi: 10.1152/jappl.1950.3.6.365. [DOI] [PubMed] [Google Scholar]
  3. BUCHANAN A. A. Lipid synthesis by human leucocytes in vitro. Biochem J. 1960 May;75:315–320. doi: 10.1042/bj0750315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAWSON R. M. Studies on the labelling of brain phospholipids with radioactive phosphorus. Biochem J. 1954 Jun;57(2):237–245. doi: 10.1042/bj0570237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAWSON R. M. The identification of two lipid components in liver which enable Penicillium notatum extracts to hydrolyse lecithin. Biochem J. 1958 Feb;68(2):352–357. doi: 10.1042/bj0680352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAWSON R. M. The measurement of 32P labelling of individual kephalins and lecithin in a small sample of tissue. Biochim Biophys Acta. 1954 Jul;14(3):374–379. doi: 10.1016/0006-3002(54)90195-x. [DOI] [PubMed] [Google Scholar]
  8. DESJOBERT A., PETEK F. Chromatographie sur papier des esters phosphoriques de l'inositol; application a l'étude de la dégradation hydrolytique de l'inositolhexaphosphate. Bull Soc Chim Biol (Paris) 1956 Sep 4;38(5-6):871–883. [PubMed] [Google Scholar]
  9. Erickson B. N., Williams H. H., Avrin I., Lee P. THE LIPID DISTRIBUTION OF HUMAN PLATELETS IN HEALTH AND DISEASE. J Clin Invest. 1939 Jan;18(1):81–85. doi: 10.1172/JCI101029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GRAY G. M., MACFARLANE M. G. Separation and composition of the phospholipids of ox heart. Biochem J. 1958 Nov;70(3):409–425. doi: 10.1042/bj0700409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HANAHAN D. J., DITTMER J. C., WARASHINA E. A column chromatographic separation of classes of phospholipides. J Biol Chem. 1957 Oct;228(2):685–700. [PubMed] [Google Scholar]
  12. HANAHAN D. J., OLLEY J. N. Chemical nature of monophosphoinositides. J Biol Chem. 1958 Apr;231(2):813–828. [PubMed] [Google Scholar]
  13. HANAHAN D. J., TURNER M. B., JAYKO M. E. The isolation of egg phosphatidyl choline by an adsorption column technique. J Biol Chem. 1951 Oct;192(2):623–628. [PubMed] [Google Scholar]
  14. HAWTHORNE J. N., HUBSCHER G. Separation of glycerylphosphoryl inositol and related compounds on ion-exchange columns. Biochem J. 1959 Jan;71(1):195–200. doi: 10.1042/bj0710195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HOKIN L. E., HOKIN M. R. Acetylcholine and the exchange of inositol and phosphate in brain phosphoinositide. J Biol Chem. 1958 Oct;233(4):818–821. [PubMed] [Google Scholar]
  16. HOKIN L. E., HOKIN M. R. Effects of acetylcholine on the turnover of phosphoryl units in individual phospholipids of pancreas slices and brain cortex slices. Biochim Biophys Acta. 1955 Sep;18(1):102–110. doi: 10.1016/0006-3002(55)90013-5. [DOI] [PubMed] [Google Scholar]
  17. HOKIN L. E., HOKIN M. R. Evidence for phosphatidic acid as the sodium carrier. Nature. 1959 Oct 3;184(Suppl 14):1068–1069. doi: 10.1038/1841068a0. [DOI] [PubMed] [Google Scholar]
  18. HOKIN L. E., HOKIN M. R. Phosphoinositides and protein secretion in pancreas slices. J Biol Chem. 1958 Oct;233(4):805–810. [PubMed] [Google Scholar]
  19. HOKIN L. E., HOKIN M. R. Studies of pancreatic tissue in vitro. Gastroenterology. 1959 Mar;36(3):368–376. [PubMed] [Google Scholar]
  20. HOKIN L. E., HOKIN M. R. The mechanism of phosphate exchange in phosphatidic acid in response to acetylcholine. J Biol Chem. 1959 Jun;234(6):1387–1390. [PubMed] [Google Scholar]
  21. HOKIN L. E., HOKIN M. R. The presence of phosphatidic acid in animal tissues. J Biol Chem. 1958 Oct;233(4):800–804. [PubMed] [Google Scholar]
  22. HUBSCHER G., CLARK B. Metabolism of phospholipids. II. Isolation and properties of phosphatidic acid from mammalian liver. Biochim Biophys Acta. 1960 Jun 17;41:45–54. doi: 10.1016/0006-3002(60)90367-x. [DOI] [PubMed] [Google Scholar]
  23. JAMES A. T., LOVELOCK J. E., WEBB J. P. The lipids of whole blood. Lipid biosynthesis in human blood in vitro. Biochem J. 1959 Sep;73:106–115. doi: 10.1042/bj0730106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. KATES M. Hydrolysis of lecithin by plant plastid enzymes. Can J Biochem Physiol. 1955 Jul;33(4):575–589. [PubMed] [Google Scholar]
  25. KENNEDY E. P. Metabolism of lipides. Annu Rev Biochem. 1957;26:119–148. doi: 10.1146/annurev.bi.26.070157.001003. [DOI] [PubMed] [Google Scholar]
  26. LOVELOCK J. E., JAMES A. T., ROWE C. E. The lipids of whole blood. 2. The exchange of lipids between the cellular constituents and the lipoproteins of human blood. Biochem J. 1960 Jan;74:137–140. doi: 10.1042/bj0740137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. MARCUS A. J., SPAET T. H. Platelet phosphatides: their separation, identification, and clotting activity. J Clin Invest. 1958 Dec;37(12):1836–1847. doi: 10.1172/JCI103776. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. MARINETTI G. V., ERBLAND J., ALBRECHT M., STOTZ E. The application of chromatographic methods to study the incorporation of 32P-labeled orthophosphate into the phosphatides of rat liver homogenates. Biochim Biophys Acta. 1957 Sep;25(3):585–591. doi: 10.1016/0006-3002(57)90531-0. [DOI] [PubMed] [Google Scholar]
  29. MARINETTI G. V., ERBLAND J., KOCHEN J. Quantitative chromatography of phosphatides. Fed Proc. 1957 Sep;16(3):837–844. [PubMed] [Google Scholar]
  30. MARINETTI G. V., WITTER R. F., STOTZ E. The incorporation in vivo of P32-labeled orthophosphate into individual phosphatides of rat tissues. J Biol Chem. 1957 May;226(1):475–483. [PubMed] [Google Scholar]
  31. PHILLIPS G. B. The isolation and quantitation of the principle phospholipid components of human serum using chromatography on silicic acid. Biochim Biophys Acta. 1958 Sep;29(3):594–602. doi: 10.1016/0006-3002(58)90017-9. [DOI] [PubMed] [Google Scholar]
  32. ROWE C. E. The biosynthesis of phospholipids by human blood cells. Biochem J. 1959 Nov;73:438–442. doi: 10.1042/bj0730438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. STRICKLAND E. H., BENSON A. A. Neutron activation paper chromatographic analysis of phosphatides in mammalian cell fractions. Arch Biochem Biophys. 1960 Jun;88:344–348. doi: 10.1016/0003-9861(60)90247-2. [DOI] [PubMed] [Google Scholar]
  34. TROUP S. B., REED C. F., MARINETTI G. V., SWISHER S. N. Thromboplastic factors in platelets and red blood cells: observations on their chemical nature and function in vitro coagulation. J Clin Invest. 1960 Feb;39:342–351. doi: 10.1172/JCI104045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. WADE H. E., MORGAN D. M. Detection of phosphate esters on paper chromatograms. Nature. 1953 Mar 21;171(4351):529–530. doi: 10.1038/171529a0. [DOI] [PubMed] [Google Scholar]
  36. WALLACH D. F. H., SURGENOR D. M., STEELE B. B. Calcium-lipid complexes in human platelets. Blood. 1958 Jun;13(6):589–598. [PubMed] [Google Scholar]

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