Skip to main content
. 2010 Jul 20;5(7):e11665. doi: 10.1371/journal.pone.0011665

Figure 4. Src and PI3K act in a linear signaling pathway.

Figure 4

A, F-actin colocalization with spores is reduced in the presence of WM, LY and PP2. A549 cells were pre-incubated with WM (100 nM), LY (50 µM), PP2 (10 µM), PP3 (10 µM), LY (50 µM) + PP2 (10 µM) or solvent control for 1 hr and then incubated with Texas Red-labeled 7702 spores in the presence of the respective inhibitors for 30 min and then stained for F-actin. The samples were examined using confocal fluorescence microscopy. Relative F-actin colocalization is the ratio of spores that associated with enriched F-actin staining vs. total spores that attached to the cells, normalized to the solvent control. B, Akt-PH-GFP recruitment is reduced in the presence of LY and PP2. A549 cells transfected with Akt-PH-GFP were pre-incubated with LY (50 µM), PP2 (10 µM), PP3 (10 µM), LY (50 µM) + PP2 (10 µM) or solvent control for 1 hr and then incubated with labeled spores in the presence of the respective inhibitors for 8 min. Relative Akt-PH-GFP recruitment is the ratio of spores that recruited Akt-PH-GFP vs. total spores that attached to the cells, normalized to the solvent control. The results are the mean ± SEM from at least three experiments. ***, p<0.001; **, p<0.01, t test.