Figure 5. Src activity is required for B. anthracis translocation through an A549 cell barrier.

A549 cells grown in transwell inserts were pre-treated with PP2 (10 µM), PP3 (10 µM), and SU6656 (50 µM) or solvent (Ctrl) for 1 hr. Spores were then added to the top chambers as described in Materials and Methods. Media was collected from the bottom chambers after 16 hr of incubation. An aliquot of the media was dilution plated to determine bacterial counts (A). Another aliquot was used to measure fluorescence emission at 520 nm (FITC-dextran) (B). The effect of the inhibitors on bacterial growth was also determined (C) as described in Materials and Methods. The results shown are mean ± SEM from at least two independent experiments. ***, p<0.001, t test.