Figure 6. Src activity is important for bacterial dissemination in vivo.
A/J mice were treated with SU6656 (7.5 µg/g body weight) or equivalent volumes of solvent 24 hr prior to spore inoculation as described in Materials and Methods. A – C, bacterial burden in the spleen (A), blood (B) and lungs (C) of mice inoculated by intranasal instillation. Each mouse was inoculated with 1 - 3×106 spores. Organs were harvested at 72 hr post inoculation, homogenized and plated. The results are combined from 2 – 3 independent experiments with a total of 7–15 mice per treatment group. D, mice were inoculated with 1×105 spores/mouse by i.p. injection. Bacterial burden in the spleen was determined at 72 hr post inoculation. The results are combined from 2 independent experiments with a total of 12 mice per treatment group. E, mice were inoculated with 1×104 spores/mouse by injection into the tail vein. Bacterial counts in the blood were determined at 84 hr post inoculation. The results were combined from 2 independent experiments with a total of 13 mice per treatment group. Statistical significance was calculated using t test. *, p<0.05. F, SU6656 treatment improves survival in i.n. inoculated mice. Mice were inoculated with ∼8×106 spores/mouse intranasally (10 mice per group). Statistical significance was calculated using the Logrank test. Similar trend was observed in three other experiments. G, mice were inoculated with ∼1×105 spores/mouse by i.p. injection. The results are from two experiments with a total of 20 mice per group.