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. 2010 Jul 20;5(7):e11660. doi: 10.1371/journal.pone.0011660

Figure 3. CIB1 is dispensable for immunoglobulin gene conversion in DT40.

Figure 3

A. Schematic showing the constructs used to replace exons 5 and 6 of CIB1 with the indicated drug resistance cassettes. The positions of the allele-specific PCR primers for genotyping and the XhoI sites and the position of the 3′ external probe used for Southern blot analysis are shown. B. An agarose gel image showing the allele-specific PCR products from CIB1+/+, +/−, and −/− cell lines. C. An agarose gel image of CIB1-specific RT-PCR products from CIB1+/+, +/−, and −/− cell lines. AID-specific reactions were used to demonstrate the integrity of each cDNA preparation. D. An IGC fluctuation analysis showing the percentage of surface Ig-positive cells in subclone cultures of the indicated genotype. Each X represents data from an individual subclone and the labeled horizontal bars report the medians for each data set.