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. 2010 Jul 20;5(7):e11660. doi: 10.1371/journal.pone.0011660

Figure 5. CIB1 is dispensable for CSR in mice.

Figure 5

A. Relative levels of each antibody isotype in sera from CIB1+/+ or CIB1−/− mice as measured by ELISA. The CIB1−/− data were normalized to the mean antibody levels in sera from wildtype (WT) littermates (arbitrarily set to 1 for comparison). Each X represents data from an independent animal and the horizontal bars and labels report the median values (n = 3 for CIB+/+ and n = 6 for CIB−/−). B. IgM to IgG1 CSR ex vivo. B-cells were isolated from the spleens of CIB1+/+ or CIB1−/− mice, cultured for 4 days in the presence of LPS and IL-4, and analyzed by α-IgG1-PE labeling and flow cytometry. Each X represents data from an independent animal and the horizontal bars and labels report the median values. C. Images of hematoxylin and eosin stained sections of spleen isolated from CIB1+/+ and CIB1−/− mice. Scale bars indicate 500 µm.