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Amplification products were generated using PCR primers flanking the Ser-rich region between the second and third chitin-binding domains of Jacob2. A. Jacob2 PCR products from axenized Eh isolates (HM-1:IMSS, HK-9, 200:NIH, and SD157) and Ed isolate (SAW760) have distinct mobilities on agarose gels. B. Jacob2 PCR products from clinical Eh isolates also have distinct mobilities.
