Table 1. Housekeeping genes and primers used in the O. tsutsugamushi MLST scheme.

Gene	Gene name	Primer	1st PCR	Product	Primer	2nd PCR	Product	MLST fragment
			sequence (5′->3′)	size (bp)		sequence (5′->3′)	size (bp)	size (bp)
gpsA	glycerol-3-phosphate dehydrogenase	gpsA_F	TCAGCCCATACTCAAGAAATCA	572	gpsA_NF	TCAGCTGCATACTAATAAAAA	510	390
		gpsA_R	GCAAATGCCACAATTTCCTT		gpsA_NR	GATGCTTTACAGTTTTGACCA
mdh	malate dehydrogenase	mdh_F	CCAAAGCAGTTGCTCAAGGT	608	mdh_NF	AAAGCATGGGTATTGGTAAA	512	348
		mdh_R	AGCTGCTGCTGGAGCATAAT		mdh_NR	TCCTCCATCTCTAGTTCTTTGT
nrdB	ribonucleoside-diphosphate reductase	nrdB_F	TAAAGCATGGCACACTCAGC	595	nrdB_NF	AAATTCACTGGCTACCAGAA	500	384
	beta subunit	nrdB_R	CTGTTCTGTCCAAACTTCAGGA		nrdB_NR	TGTTTCATCTCTAACTGACCA
nuoF	NADH dehydrogenase chain F	nuoF_F	ATCTGGTTCTATGGCAGTTGAC	645	nuoF_NF	AAAATCTGGCTTACGTGGT	520	360
		nuoF_R	CATTTTGCGCCTCTTCTGAGTA		nuoF_NR	GAGTATTGTCGGAACTACAGC
ppdK	pyruvate, phospate dikinase precursor	ppdK_F	CAAAGGTGTAACACTTGCTCAGA	591	ppdK_NF	TACCTATACCGCATGGTTTT	528	396
		ppdK_R	TGGTGGTTCATCCATGATTTT		ppdK_NR	ACTGCTTGAATAGCTTGGTG
sucB	dihydrolipoamide S-succinyltransferase	sucB_F	CAGCAAAAGAAAGATGTTCAGC	590	sucB_NF	ATTGGCACAACTAATCCAGA	537	411
		sucB_R	GGTTGCCAAAATGGTAGCAG		sucB_NR	GCATAAAATCAATCCTGAGAA
sucD	succinyl-CA synthase alpha chain	sucD_F	ATGTTCCTCCAGCTTTTGCT	599	sucD_NF	TGAAGCTATTGATGCTGGTA	562	411
		sucD_R	TCCAGCGCTTTTTAATGCTT		sucD_NR	AGCGCTTTTTAATGCTTCTA