Fig. 1.

Phenotypic characterization of the ΔdipM mutant. (A) Growth rates (in doublings/h) of wild-type and ΔdipM (CJW3137) CB15N strains grown at 30°C in liquid cultures containing M2G minimal medium, M2G medium supplemented with 15% of rich PYE medium, or PYE medium. Average values and standard deviations for three experiments are shown. (B) Phase contrast images of ΔdipM cells grown at 22°C in different media. ΔdipM cells from overnight M2G cultures at 30°C were used to inoculate culture tubes containing M2G medium, PYE medium or M2G supplemented with 10 or 15% of PYE medium, which were then incubated at 22°C for an additional 24 h prior to imaging. At the time of imaging, the cultures were at an OD₆₆₀ ≤ 0.5. (C) Scanning EM images of ΔdipM mutant cells grown at 30°C in liquid M2G medium. White and black arrows indicate constriction sites and bulged poles, respectively. For comparison, the inset shows scanning EM images of a wild-type predivisional cell grown under the same conditions.