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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Insect Mol Biol. 2010 Apr 27;19(4):423–429. doi: 10.1111/j.1365-2583.2010.00999.x

Figure 2.

Figure 2

Quantitative rt-PCR for the expression of two groups of Culex tarsalis vitellogenin genes (Vg1a and b, Vg2a and b) during (a) immature development and (b) autogeny in KNWR-au. RNA for cDNA synthesis was extracted from ~200 newly hatched/first day, ~50-100 second day, 20 fourth day and 10 sixth day after hatching larvae, 10 females during each pupal stage, 10 females following emergence at indicated time points. QRT-PCR was performed with primers specific to either Vg1a and b or Vg2a and b. Data were normalized to actin expression. Each point represents the average of three replicates and the bars are the standard error of the mean. Note the difference in scale between a and b.