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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Insect Mol Biol. 2010 Apr 27;19(4):423–429. doi: 10.1111/j.1365-2583.2010.00999.x

Figure 3.

Figure 3

a. Expression of the four vitellogenin genes during larval and pupal development in KNWR-au. RNA for cDNA synthesis was extracted from groups of 10 females collected at indicated time points (0h and every 12 hours out to 96 hours) following eclosion from the pupal stage. RT-PCR was performed using primers specific to each of the four Cx. tarsalis vitellogenin genes (Vg1a, Vg1b, Vg2a, Vg2b) and actin to control for RNA quality. The experiment was repeated three times. b. Western blot analysis of vitellogenin production during larval and pupal development in KNWR-au. Protein was extracted from groups of 10 individuals as 4th instar larvae, 0, 12, 24, 36 hour pupae and 5 vitellogenic adult Cx. tarsalis females. Vitellogenin was detected using an antibody to the 68kD small subunit of Ae. aegypti vitellogenin. c. Coomassie blue stained gel of separated Cx. tarsalis pupal proteins and protein from vitellogenic adult Cx. tarsalis females.