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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Cell Biochem. 2010 Aug 1;110(5):1195–1207. doi: 10.1002/jcb.22634

Figure 1. Adiponectin activates AMPKα2 in activated HSCs.

Figure 1

A). Western blot of the total and active phosphorylated forms of AMPK, and β-actin in lysates prepared from leptin-treated rat HSCs. Leptin treatment alone caused weak increases in AMPK phosphorylation at all time points examined. B). Western blot of the total and active phosphorylated forms of AMPK in lysates prepared from adiponectin-treated rat HSCs demonstrating that adiponectin increased AMPK phosphorylation ~5 fold by 5 min and 3.2-fold at 30 min (*p < 0.05 vs. leptin). C). Co-administration of leptin and adiponectin resulted in a 3.8-fold increase in AMPK phosphorylation at 5min (*p < 0.05 vs. leptin or adiponectin alone), and 3.2-fold at 30 min, (*p < 0.05 vs. leptin). The blots are representative of three independent experiments performed in triplicate.