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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Cell Biochem. 2010 Aug 1;110(5):1195–1207. doi: 10.1002/jcb.22634

Figure 4. Adiponectin decreases tissue inhibitor of metalloproteinase (TIMP)-1 secretion in the presence of leptin and increases MMP-1 activity.

Figure 4

A) Western analysis of extracellular TIMP-1 from leptin (Lep), adiponectin (Adi), and co-treated (LA) rat HSCs. Ponceau S staining of the membranes was used as a protein loading control. B) Western analysis of intracellular TIMP-1 from leptin (Lep), adiponectin (Adi), and co-treated (LA) rat HSCs. β-actin was used as a protein loading control. (C) Quantitative representation of the relative intracellular (white bars), extracellular (gray bars) TIMP-1 protein levels, and the ratio of extracellular to intracellular (extra:intra, black bars) TIMP-1 protein from leptin (Lep), Adiponectin (Adi), or leptin and adiponectin (LA)-treated rat HSCs, D) Relative MMP-1 activity in rat HSCs was assessed by fluorimetric immunoassay. Adiponectin alone (Adi) or the co-administration of adiponectin and leptin (LA) resulted in a significant increase in MMP-1 activity compared to untreated samples (Utx) or leptin treatment alone (Lep) (**p < 0.01 vs. untreated).