Table 2.
Identification of various proteins upregulated by redox stress in hepatocytes via redox proteomics. Reprinted with permission from Vascotto C, Cesaratto L, D’Ambrosio C, Scaloni A, Avellini C, Paron I, Baccarani U, Adani GL, Tiribelli C, Quadrifoglio F, Tell G. Proteomic analysis of liver tissues subjected to early ischemia/reperfusion injury during human orthotopic liver transplantation. Proteomics. 6:3455–3465,2006. Copyright Wiley-VCH Verlag GmbH & Co. KGaA. [171]
| Protein Number | Protein identity | Swiss-Prot entry | MW | P/ | % Seguence coverage | T2 vs T1, ratio | #of patients/9 | Function | Subcellular localization |
|---|---|---|---|---|---|---|---|---|---|
| a) Lipid metabolism | |||||||||
| 1 | 3,2 trans enoyl CoA isomerase | P42126 | 35 (33) | 5.8 (8.8) | 39 | 4.79 | 2 | Lipid metabolism | Mitochondrial |
| 2 | Apolipoprotein A1 | P02647 | 30 (31) | 5.0 (5.6) | 38 | 14.45 | 2 | Lipid metabolism | Plasma |
| 3 | ACADV very long chain | P49748 | 70 (70) | 7.4 (8.9) | 19 | 1.85 | 1 | Lipid metabolism | Mitochondrial |
| 4 | HCD2 | Q99714 | 30 (27) | 6.9 (7.7) | 34 | 2.09 | 1 | Lipid metabolism | Membrane |
| 5 | Long-chain-fatty acid CoA ligase 1 | P33121 | 80 (78) | 6.4 (6.8) | 32 | 0.42 | 1 | Lipid metabolism | Membrane |
| b) Energy metabolism | |||||||||
| 6 | Fructose-biphosphate aldolase B | P05062 | 40 (39) | 5.9 (8.1) | 49 | 5.68 | 3 | Glycolysis | Cytoplasmic |
| 7 | G3P2 | P04406 | 16 (36) | 5.9 (8.6) | 29 | Q | 1 | Glycolysis | Cytoplasmic |
| 8 | Methyl malonate semialdehyde dehydrogenase | Q02252 | 30 (58) | 6.2 (8.7) | 13 | Q | 1 | Metabolic pathways | Mitochondrial |
| 9 | ODPB component beta subunit | P11177 | 40 (39) | 5.3 (6.2) | 26 | 4.37 | 1 | Tricarboxylic acid cycle | Mitochondrial |
| 10 | KHK isoform C | P50053 | 35 (33) | 5.6(5.6) | 39 | 4.07 | 1 | Dietary fructose metabolism | Cytoplasmic |
| 11 | Phosphoglucomutase | P36871 | 85 (61) | 6.0 (6.3) | 22 | 0.24 | 1 | Glucose metabolism | Cytoplasmic |
| 12 | ETFB beta subunit | P38117 | 30 (28) | 8.0 (8.2) | 35 | 3.61 | 1 | Electron transport | Mitochondrial |
| 13 | CYB5 | P00167 | 16 (15) | 4.5 (4.9) | 49 | Q | 1 | Electron transport | ER |
| c) Redox | |||||||||
| 14 | GST A1 | P08263 | 18 (25) | 7.4 (8.9) | 35 | 9.88 | 3 | Conjugation of reduced glutathione | Cytoplasmic |
| 15 | PRDX5 | P30044 | 20 (22) | 6.6 (8.8) | 36 | 1.92 | 1 | Peroxidase activity | Mitochondrial, peroxisomal an Cytoplasmic |
| 16 | GST A2 | P09210 | 30 (26) | 8.5 (8.5) | 36 | 2.14 | 1 | Conjugation of reduced glutathione | Cytoplasmic |
| 17 | BLVRB | P30043 | 25 (22) | 7.1 (7.3) | 62 | 1.87 | 1 | Biliverdin reductase activity | Cytoplasmic |
| 18 | ADHA alpha chain | P07327 | 25 (40) | 8.0 (8.6) | 20 | 9.61 | 5 | Alcohol oxidation | Cytoplasmic |
| 19 | ADH4 | P08319 | 45 (42) | 7.2 (8.2) | 16 | 4.27 | 1 | Alcohol oxidation | Cytoplasmic |
| d) Protein and aminoacid metabolism | |||||||||
| 20 | SPYA | P21549 | 30 (43) | 5.9 (8.6) | 24 | 2.75 | 2 | Aminotransferase activity | Peroxisomal |
| 21 | 4-Hydroxyphenylpiruvate dioxygenase | P32754 | 50 (45) | 6.1 (6.5) | 32 | 0.46 | 2 | Tyr, Phe catabolism | Cytoplasmic |
| 22 | Ornithine carbamoyl transferase | P00480 | 45 (40) | 7.0 (8.8) | 20 | Q | 1 | Urea cycle, Arg biosynthesis | Mitochondrial |
| 23 | 3-Phosphoglycerate dehydrogenase | Q43175 | 65 (57) | 6.0 (6.3) | 28 | 0.34 | 1 | Ser biosynthesis | Cytoplasmic |
| e) Molecular chaperone | |||||||||
| 24 | Peptidyl-prolyl cis-trans iso- merase A (Cyclophilin A) | P05092 | 20 (18) | 7.0 (7.8) | 29 | 1.74 | 1 | Protein folding | Cytoplasmic |
| 25 | Heat shock cognate 71 kDa protein | P11142 | 20 (71) | 6.0 (5.4) | 13 | Q | 1 | Molecular chaperone | Cytoplasmic-nuclear |
| f) Metabolic pathway | |||||||||
| 26 | Liver carboxyl esterase | P23141 | 65 (63) | 5.9 (6.1) | 23 | 7.64 | 2 | Metabolic pathways | EB |
| 27 | CAH1 | P00915 | 35 (29) | 6.5 (6.6) | 43 | 3.47 | 2 | Carbonate dehy dratase activity | Cytoplasmic |
| 28 | C-1- tetrahydrofolate synthase | P11586 | 115 (101) | 6.9 (6.9) | 20 | 2.00 | 1 | Biosynthetic pathways | Cytoplasmic |
| 29 | G6PE | Q95479 | 100 (89) | 6.9 (6.8) | 25 | 1.71 | 1 | Metabolic pathways | ER |
| 30 | Carbonyl reductase | Q9UHY9 | 30 (26) | 7.8 (8.3) | 39 | 1.99 | 1 | Metabolic pathways | Membrane |
| 31 | CAH2 | P00918 | 28 (29) | 6.1 (6.9) | 40 | 6.26 | 1 | Carbonate dehydratase activity | Cytoplasmic |
| 32 | FTHFD | Q75891 | 115 (99) | 5.6 (5.6) | 41 | 0.41 | 1 | Metabolic pathways | Cytoplasmic |
| 33 | Aldo-keto reductase family 1 member C1 | Q04828 | 35:371 | 6.1 (8.0) | 22 | Q | 1 | Metabolic pathways | Cytoplasmic |
| g) Secreted proteins | |||||||||
| 34 | HSA | P02768 | 85 (69) | 5.6 (5.9) | 43 | 9.15 | 3 | Transporter activity | Plasma |
| h) Inflammatory response | |||||||||
| 35 | S10AS | P05109 | 14 (11) | 6.5 (6.5) | 33 | 7.59 | 1 | Calcium ion binding, inflammatory response | Cytoplasm of macrophages |
| i) unknown | |||||||||
| 36 | ES1 protein homologue | P30042 | 30 (28) | 6.6 (8.5) | 55 | 2.08 | 1 | Unknown | Mitochondrial |