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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5504–5510. doi: 10.1021/bi100433x

Table 3.

Kinetic parameters for non-natural nucleotide analog incorporation into D-G DNA catalyzed by hRev1 at 37 °C.

dNTP kp (s-1) Kd (μM) kp/Kd (μM-1s-1) Efficiency Ratioa
dCTP 22.4 ± 0.9 2.2 ± 0.3 10
dATP 0.050 ± 0.004 70 ± 20 7.1 × 10-4 1.4 × 104
dNITP 0.0457 ± 0.0006 15.8 ± 0.6 2.9 × 10-3 3.5 × 103
dPTP 0.0228 ± 0.0008 25 ± 3 9.1 × 10-4 1.1 × 104
a

Calculated as (kp/Kd)dCTP/(kp/Kd)dNTP.