Figure 6.

Up-regulation of HIF-1α inhibits poly(I:C)-induced apoptosis in LNCaP and PC3 cells. PC3 cells transfected with scramble or hif-1α interfering plasmids, treated or not with 5 µg/ml poly(I:C) for 24 hours, were analyzed for the apoptotic rate using PI staining (A) and caspase-3 activation (B). LNCaP cells overexpressing I.3 isoform of hif-1α were treated with 5 µg/ml poly(I:C) for 24 hours, and PI staining (C) and caspase-3 activation (D) were performed to evaluate the apoptotic rate. Histograms in panels A and C represent the mean of triplicate samples from three independent experiments with SD as error bars, **P ≤ .01. Data shown in panels B and D are typical experiments repeated three times with similar results.