Table 5.
RpoS negatively regulated genes on the O-islands (P < 0.05). MER: mean expression ratio (rpoS/WT).
| Gene | Expression (log2) | MER | Position | Function | |
|---|---|---|---|---|---|
| WT | rpoS | ||||
| Z0264 | 7.8 ± 0.1 | 9.0 ± 0.0 | -2 | O-Island 7 | Unknown |
| Z0372 | 11.4 ± 0.3 | 12.6 ± 0.2 | -2 | O-Island 11 | Unknown |
| Z0397 | 5.1 ± 0.3 | 6.2 ± 0.1 | -2 | O-Island 14 | Unknown |
| Z0955 | 9.7 ± 0.3 | 11.5 ± 0.0 | -4 | O-Island 36 | Unknown (prophage CP-933K) |
| Z1146 | 11.7 ± 0.3 | 12.7 ± 0.3 | -2 | O-Island 43 | Putative urease accessory protein E |
| Z1144 | 11.3 ± 0.2 | 12.4 ± 0.2 | -2 | O-Island 43 | Putative urease structural subunit B |
| Z1142 | 10.9 ± 0.3 | 12.1 ± 0.2 | -2 | O-Island 43 | Putative urease accessory protein D |
| Z1164 | 12.1 ± 0.1 | 13.4 ± 0.0 | -2 | O-Island 43 | Unknown |
| Z1143 | 10.9 ± 0.3 | 12.3 ± 0.2 | -3 | O-Island 43 | Putative urease structural subunit A |
| Z1160 | 3.7 ± 0.1 | 5.5 ± 0.4 | -4 | O-Island 43 | Unknown |
| Z1163 | 7.5 ± 0.5 | 9.4 ± 0.4 | -4 | O-Island 43 | Unknown |
| Z1346 | 11.9 ± 0.1 | 13.0 ± 0.2 | -2 | O-Island 44 | Unknown (cryptic prophage CP-933M) |
| Z1348 | 10.8 ± 0.1 | 11.9 ± 0.2 | -2 | O-Island 44 | Unknown (cryptic prophage CP-933M) |
| Z1324 | 4.4 ± 0.1 | 5.8 ± 0.3 | -3 | O-Island 44 | Putative exoDNaseVIII |
| Z1347 | 10.0 ± 0.0 | 11.5 ± 0.2 | -3 | O-Island 44 | Unknown (cryptic prophage CP-933M) |
| Z1326 | 3.4 ± 0.3 | 5.5 ± 0.3 | -4 | O-Island 44 | Putative inhibitor of cell division |
| Z1325 | 4.1 ± 0.4 | 6.3 ± 0.3 | -5 | O-Island 44 | Unknown (cryptic prophage CP-933M) |
| Z1456 | 12.8 ± 0.2 | 13.8 ± 0.3 | -2 | O-Island 45 | Unknown (bacteriophage BP-933W) |
| Z1503 | 8.0 ± 0.5 | 10.2 ± 0.5 | -4 | O-Island 45 | Unknown (bacteriophage BP-933W) |
| Z1794 | 5.6 ± 0.3 | 6.8 ± 0.3 | -2 | O-Island 50 | Putative holin protein |
| Z1878 | 13.0 ± 0.2 | 14.7 ± 0.1 | -3 | O-Island 52 | Putative Bor protein |
| Z2146 | 5.8 ± 0.2 | 7.0 ± 0.1 | -2 | O-Island 57 | Putative OMP Lom precursor |
| Z2100 | 2.4 ± 0.1 | 3.7 ± 0.2 | -2 | O-Island 57 | Unknown (prophage CP-933O) |
| Z2045 | 9.9 ± 0.1 | 11.4 ± 0.1 | -3 | O-Island 57 | Regulator of DicB |
| Z2105 | 8.8 ± 0.2 | 10.3 ± 0.1 | -3 | O-Island 57 | Unknown (prophage CP-933O) |
| Z2101 | 3.8 ± 0.0 | 5.3 ± 0.3 | -3 | O-Island 57 | Putative endonuclease |
| Z2103 | 10.5 ± 0.1 | 12.0 ± 0.1 | -3 | O-Island 57 | Unknown (prophage CP-933O) |
| Z2144 | 5.9 ± 0.2 | 7.6 ± 0.2 | -3 | O-Island 57 | Putative tail component of CP-933O |
| Z2059 | 5.3 ± 0.3 | 8.7 ± 0.3 | -11 | O-Island 57 | Unknown (prophage CP-933O) |
| Z2510 | 5.0 ± 0.4 | 7.0 ± 0.2 | -4 | O-Island 70 | Putative transcriptional repressor |
| Z3201 | 12.0 ± 0.3 | 13.2 ± 0.2 | -2 | O-Island 84 | O antigen flippase Wzx |
| Z3361 | 7.3 ± 0.2 | 8.3 ± 0.1 | -2 | O-Island 93 | Putative regulatory protein |
| Z3360 | 11.8 ± 0.1 | 13.0 ± 0.2 | -2 | O-Island 93 | Unknown (prophage CP-933V) |
| Z3322 | 5.0 ± 0.2 | 6.3 ± 0.2 | -2 | O-Island 93 | Putative major tail subunit |
| Z3622 | 6.9 ± 0.2 | 10.1 ± 0.7 | -9 | O-Island 102 | Putative resolvase |
| Z4048 | 8.4 ± 0.2 | 10.4 ± 0.1 | -4 | O-Island 110 | Putative regulator |
| Z4789 | 3.1 ± 0.2 | 4.4 ± 0.1 | -2 | O-Island 133 | Unknown |
| Z4851 | 7.4 ± 0.0 | 8.6 ± 0.2 | -2 | O-Island 138 | Unknown |
| Z4855 | 9.4 ± 0.2 | 10.5 ± 0.1 | -2 | O-Island 138 | Unknown |
| Z4852 | 8.9 ± 0.2 | 10.1 ± 0.1 | -2 | O-Island 138 | Putative acyltransferase |
| Z4857 | 3.5 ± 0.3 | 4.9 ± 0.3 | -3 | O-Island 138 | Unknown |
| Z4854 | 8.7 ± 0.3 | 10.2 ± 0.1 | -3 | O-Island 138 | Putative acyl carrier protein |
| Z4861 | 3.2 ± 0.5 | 5.7 ± 0.4 | -6 | O-Island 138 | Unknown |
| Z4860 | 6.3 ± 0.3 | 8.8 ± 0.2 | -6 | O-Island 138 | Unknown |
| Z5051 | 10.2 ± 0.3 | 11.4 ± 0.1 | -2 | O-Island 145 | Putative LPS biosynthesis enzyme |
| Z5049 | 11.7 ± 0.3 | 13.5 ± 0.3 | -3 | O-Island 145 | Putative LPS biosynthesis enzyme |
| Z5089 | 3.8 ± 0.2 | 4.9 ± 0.1 | -2 | O-Island 148 | Putative transposase |
| Z5110 | 7.6 ± 0.2 | 8.9 ± 0.1 | -2 | O-Island 148 | LEE-encoded virulence protein Eae |
| Z5225 | 3.6 ± 0.2 | 4.7 ± 0.2 | -2 | O-Island 154 | Putative major fimbrial subunit |
- Indicates negative regulation.