Table 4.
Semi Quantitative Real Time PCR Results
| Affymetrix Probe Set | Annotation | Conditions of Differential Expression | Differential Expression in Microarray | Diff Express in sqRT-PCR |
|---|---|---|---|---|
| Gma.13296.3.S1_at | Lipid Transfer | CSSvCSD | 190.34 | 2.62 |
| Gma.17724.3.S1_at | GDSL motif | CSSvCSD | 6.26 | 8.46 |
| Gma.17825.1.A1_at | GDSL motif | CSSvCSD | 4.69 | 10.26 |
| GmaAffx.89896.1.S1_at | Heat Shock Protein | CSSvCSD | 23.45 | 2.04 |
| GmaAffx.93268.1.S1_at | Heat Shock Protein | CSSvCSD | 19.91 | 1.36 |
| GmaAffx.51733.1.A1_at | Ribonuclease T2 | CSSvCSD | -13.35 | -2.98 |
| GmaAffx.88242.1.S1_at | SnRNP protein | CSSvCSD | 3.58 | 11.18 |
| Gma.16500.1.S1_at | Lipoxygenase | CSSvCSD | 11.36 | 16.9 |
| Gma.3705.1.S1_at | Nitrate Reductase | CSDvICSD | -3.53 | -1.101 |
| Gma.9609.1.S1_at | Reductase | CSDvICSD | 2.26 | 2.23 |
| GmaAffx.36066.1.S1_at | Replication Factor | CSDvICSD | 2.63 | 3.58 |
Details of sqRT-PCR experiments. Affymetrix Probe set denotes the differentially expressed sequence for which primers were designed. Conditions of Differential Expression represent the genotype and growth conditions in the comparison where the sequence was differentially expressed (CSS – Clark Shoots Iron Suficient, CSD – Clark Shoots Iron Deficient, ICSD – IsoClark Shoots Iron Deficient). Differential Expression in Microarray, the change in gene expression for the sequence from the microarray (in fold change). Differential Expression in sqRT-PCR, the change in gene expression for the sequence seen in the sqRT-PCR experiment (in fold change).