Table 6.
Clusters of Candidate Genes on 7X build of Soybean Genome from IsoClark Genotype assuming 36% of the genome is heterochromatic.
| 1,000,000 base bins | ||||
|---|---|---|---|---|
| # of Genes | Stimulation Mean | Stimulation SD | Experimental Data | SDs from Sim Mean |
| 0 | 757 | 4.98 | 783 | 5.22 |
| 1 | 165 | 8.81 | 118 | -5.33 |
| 2 | 33 | 4.40 | 38 | 1.14 |
| 3 | 4 | 2.02 | 8 | 1.98 |
| 4 | 0 | 0.66 | 7 | 10.60 |
| 100,000 base bins | ||||
| # of Genes | Stimulation Mean | Stimulation SD | Experimental Data | Sds from Sim Mean |
| 0 | 9359 | 2.13 | 9325 | -15.96 |
| 1 | 236 | 4.21 | 178 | -13.78 |
| 2 | 5 | 2.07 | 28 | 11.11 |
| 3 | 0 | 0.25 | 4 | 16 |
| 100 base bins | ||||
| # of Genes | Stimulation Mean | Stimulation SD | Experimental Data | Sds from Sim Mean |
| 1 | 95754 | 0.72 | 95128 | -869.44 |
| 1 | 245 | 1.44 | 199 | -31.94 |
| 2 | 1 | 0.72 | 22 | 29.17 |
| 3 | 0 | 0.03 | 1 | 33.33 |
Details of sqRT-PCR experiments. Affymetrix Probe set denotes the differentially expressed sequence for which primers were designed. Conditions of Differential Expression represent the genotype and growth conditions in the comparison where the sequence was differentially expressed (CSS – Clark Shoots Iron Suficient, CSD – Clark Shoots Iron Deficient, ICSD – IsoClark Shoots Iron Deficient). Differential Expression in Microarray, the change in gene expression for the sequence from the microarray (in fold change). Differential Expression in sqRT-PCR, the change in gene expression for the sequence seen in the sqRT-PCR experiment (in fold change).