Skip to main content
. 2010 Jul 14;429(Pt 3):473–483. doi: 10.1042/BJ20100155

Figure 2. The fs230 mutant is predominantly localized within the endoplasmic reticulum and reduces the number of endogenous gap junctions and the extent of GJIC.

Figure 2

(A) fs230-expressing REKs were labelled with antibodies specific for PDI (endoplasmic reticulum-resident protein) or gp130 (Golgi marker). The bulk of fs230 co-localized primarily with PDI. Nuclei were stained with DAPI (blue). (B) Both vector control and fs230-expressing REKs were immunolabelled with a C-terminal specific anti-Cx43 antibody that detected only endogenous Cx43. Arrows indicate plaque formation. (C) fs230-expressing REKs exhibited a 34% decrease in endogenous Cx43 plaque formation when compared with vector control REKs. (D) Lucifer Yellow dye was microinjected into both vector control and fs230-expressing REKs. All vector control REKs passed Lucifer Yellow dye, whereas only 33% of fs230-expressing REKs passed dye to neighbouring cells. *P<0.05.