Figure 3. Cx43 mutants reduce dye transfer in REKs.

(A) To assess the ability of REKs to transfer dye to neighbouring cells, a scrape assay was performed using a gap junction permeable dye, sulforhodamine. (B) With the exception of Δ244*, there was a significant reduction in dye spread in mutant expressing REKs compared with Cx43-overexpressing REKs. V, empty vector. *P<0.05.