Figure 6. Cx43 mutant-expressing REKs exhibited reduced transepithelial resistance when compared with Cx43 overexpressing REKs in the absence of changes in the expression or localization of junctional proteins.

(A) Transepithelial resistance measurements of confluent monolayer cultures were taken daily for 5 days and the resistance from days 2–4 were averaged and plotted. (B) E-cadherin, claudin1 and occludin were localized by immunofluorescent labelling in REKs overexpressing GFP-tagged Cx43, G138R or fs260. Western blot analysis did not reveal any changes in the expression of occludin, E-cadherin or claudin-1 (C and D). *P<0.05. Nuclei were labelled with DAPI (blue). GAPDH, glyceraldehyde-3-phosphate dehydrogenase