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. 2010 Jul 14;429(Pt 3):485–495. doi: 10.1042/BJ20091956

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© 2010 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dense granule fractions (DF) were homogenized in GluIM buffer and tested for PPX and PPase activities as described in the Materials and methods section. (A) Levels of poly P₃ and poly P₇₅₊ after different times of incubation with the dense granule fraction. (B) Levels of PP_i after different times of incubation with the dense granule fraction. Controls (Ctrl) are the substrates incubated in buffer, with no fraction added. Graphs show mean relative activities±S.E.M. for four experiments. Asterisks indicate significant differences (one-way ANOVA, P<0.05).