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Compound 1 is a substrate for PSA in vitro. Shown are HPLC traces of in vitro 6 h enzymatic reactions of 1 (500 μM) in 50 mM Tris, 0.1 M NaCl, pH 7.8, with (A) active PSA (50 μg/mL), (B) buffer only, or (C) HK PSA (50 μg/mL). The identities of the various species based on mass spectrometry are indicated on the traces. *Monoacetylated Mu-HSSKLY-PABA, **Monodeacetylated 1, and ***Isomers of 1.
