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. 2009 Mar 18;96(6):2382–2390. doi: 10.1016/j.bpj.2008.12.3916

Figure 2.

Figure 2

Extracellular space volume measurements in cell-embedded gels. (A) Brightfield micrographs of cell-embedded agarose gels containing different densities of SP2/0 cells in 0.3% agarose, giving α of 1 (gel not containing cells), 0.42, 0.21 and 0.12. (B) Time course of calcein (left panels) and SR (right panels) fluorescence after pulsed iontophoretic dye delivery into the gels. (C) Dependence of calcein (top) and SR (bottom) fluorescence, in agarose gels (at physiological pH and osmolarity), on their concentration. (D) Summary of measured α from experiments as in B versus specified α (from cell volume and density) (SE n = 4).