Figure 6.
Simulation of SR and calcein fluorescence. (A) Fluorescence intensity profiles of SR (left) and calcein (right). Data computed over 800 μm with a time step of 50 μs, distance step of 0.392 μm, α = 1, and D = 100 μm2/s. (B) Effect of distance between iontophoresis tip and fiberoptic probe on the SR (left) and calcein (right) time courses. Data were simulated as in (B) for tip-probe distances of 15, 20, and 25 μm. (C) Validation of α and D determination from calcein and SR fluorescence time course. Calcein (top) and SR (bottom) fluorescence time courses were simulated for D = 100 μm2/s and specified α. Ratios of fluorescence amplitudes and half-times, calculated by Eqs. 1 and 2 of the text, respectively, are given on the ordinate. The solid line is the line of identity.