Table 1.
Bacterial composition of cheese surface consortium F by a culture dependent method1
| Bacterial species | Accession number2 | Similarity (%) | Isolation media3 | Viable count [CFU cm-2] | Percentage on TGYA | |||||
|---|---|---|---|---|---|---|---|---|---|---|
|
Brevibacterium linens (or Brevibacterium aurantiacum4) |
GenBank:AJ315491 (GenBank:X765664) |
95.5-98.0 (97.8) |
TGYA | 7.5.108 | 32.5% | |||||
| Staphylococcus vitulinus | GenBank:NR_024670 | 99.6 | TGYA | 6.0.108 | 26.0% | |||||
| Brachybacterium tyrofermentans | GenBank:X91657 | 97.9 | TGYA | 4.5.108 | 19.5% | |||||
| Corynebacterium casei | GenBank:DQ361013 | 100.0 | TGYA | 1.5.108 | 6.5% | |||||
| Microbacterium gubbeenense | GenBank:AF263564 | 97.9 | TGYA | 1.5.108 | 6.5% | |||||
| Marinilactibacillus psychrotolerans | GenBank:AB083413 | 99.8 | TGYA | 1.5.108 | 6.5% | |||||
| Brachybacterium sp. | GenBank:AF513397 | 99.9 | TGYA | 0.7.108 | 3.0% | |||||
| Staphylococcus equorum | GenBank:NR_027520 | 98.8-99.1 | MSA | 3.0.108 | - | |||||
| Staphylococcus epidermidis | GenBank:NC_004461 | 98.5 | MSA | 8.107 | - | |||||
| Facklamia tabacinasalis | GenBank:Y17820 | 99.1 | BP | 6.105 | - | |||||
| Lactococcus lactis | GenBank:NC_002662 | 99.5 | MRS | 4.104 | - | |||||
| Enterococcus devriesei | GenBank:AJ891167 | 98.2 | MRS | 1.104 | - | |||||
| Enterococcus malodoratus | GenBank:Y18339 | 99.8 | MRS | 2.103 | - | |||||
| Enterococcus faecalis | GenBank:AJ420803 | 99.3 | KFS | 2.102 | - | |||||
| Enterococcus faecium | GenBank:EU547780 | 100.0 | KFS | 6.101 | - | |||||
1 128 isolates, i.e. ca. 25 isolates per media, were analyzed by TTGE and grouped into identical TTGE profiles. A representative isolate of each profile was identified by 16S rDNA sequencing. After the assignment of all isolates to a species, the percentage of each species on each of the five media was assessed. The cell count of a species was calculated by multiplying percentage and cell count determined for the corresponding media.
2 Closest 16S rDNA sequence in the GenBank public database http://www.ncbi.nlm.nih.gov.
3 Total cell count was determined on TGYA. In addition, staphylococci were enumerated on BP agar and MSA, lactic acid bacteria on MRS agar and enterococci on KFS agar.
4 Given the polymorphy in the intraspecies diversity of B. linens (Oberreuter et al. [52]), strain assignation to B. linens or the related species B. aurantiacum based on 16S rDNA analysis only was considered not reliable.