Table 2.
Identification of non-assigned TTGE bands by excision, cloning and sequencing
| Band Designation1 | Bacterial species | Accession number2 | Similarity (%) |
|---|---|---|---|
| c | Corynebacterium variabile | GenBank:AJ783438 | 98.3 |
| f 3 | uncultured bacterium from marine sediment | GenBank:FJ717185 | 97.2 |
| m | Brachybacterium paraconglomeratum | GenBank:AJ415377 | 96.8 |
| x | Agrococcus casei | GenBank:DQ168427 | 100 |
| y | Alkalibacterium kapii | GenBank:AB294171 | 97.5 |
1 These designations are used to annotate bands from TTGE gels in figures 2 and 3.
2 Closest 16S rDNA sequence in the GenBank public database http://www.ncbi.nlm.nih.gov.
3The 16S rDNA sequence of band f exhibited highest similarity of 94% with Clostridiisalibacter paucivorans (GenBank: EF026082), a bacterium that belong to cluster XII of the Clostridium subphylum [53].