Figure 2. Percentage of ducts and solid masses in collagen matrix after BPA, BBP and E2 treatments.
MCF-10F cells were treated with 10−5M and 10−6M BPA or BPA continuously for fifteen days. As controls the cells were treated with DMSO (vehicle) or grown in regular media. Also, MCF-10F cells were treated with 70nM E2 for 24 hours, twice a week, for two weeks. Student t-test was used for comparison between treatments and control and a p≤0.01 was accepted as statistically significant. The number of tubules and solid masses grown in four wells were counted. The mean (±SD) and the statistically significant differences from the control (*) are indicated.