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Fabrication of cell-adhesive PEG hydrogels (A) by copolymerization of PEGDA and acrylic acid, followed by post-grafting of cell-adhesive peptides (CAPs) on the hydrogel surface through the reaction between the N-terminal amino groups of CAPs and the carboxyl groups provided by acrylic acid from the hydrogel. Microfabrication of patterned cell-adhesive hydrogel surfaces (B) by microcontact printing (µCP) of biotinated CAPs (Biotin-CAPs) on streptavidin-bearing PEG hydrogels.
