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. 2000 Dec 7;1:4. doi: 10.1186/1471-2156-1-4

Figure 3.

Figure 3

Two-dimensional immunoblot analysis of proteins extracted from heart and limb muscle from adult mouse. Hundred and fifty μg of protein extracts were resolved by IEF in the first dimension and by 7.5% SDS-PAGE in the second dimension. Arrows indicate the positions of the major P82,84 modified variants at pH 6.0, 5.7 and 5.4.