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(a) Opening free energy changes (ΔGop) for rU-dA/dT-dA base pairs in the RNA-DNA and DNA-DNA duplexes investigated. The experimental errors for the values in the RNA-DNA hybrid are smaller than the symbols.
(b) The free energy contributions (ΔΔGop) of individual rU-dA/dT-dA base pairs to the destabilization of the elongation complex at the transcription termination site, calculated based on the thermodynamic model (Eq. 3).
