Table 1.
Equilibrium Constants and Free Energy Changes for Base-Pair Opening in the rU-dA RNA-DNA Hybrid and dT-dA DNA Duplexes Investigated
| rU-dA RNA-DNA Hybrid | dT-dA DNA Duplex | |||||
|---|---|---|---|---|---|---|
| Base pair | Kop (×106) | ΔGopa | Base pair | Kop (×106) | ΔGopa | ΔΔGopa |
| (rU-dA)5 | 79 ± 2 | 5.30±0.01 | (dT-dA)5 | 0.5±0.2 | 8.1±0.2 | -2.8±0.2 |
| (rU-dA)6 | 462 ± 14 | 4.31±0.02 | (dT-dA)6 | 0.3±0.1 | 8.4±0.2 | -4.1±0.2 |
| (rU-dA)7 | 497 ± 19 | 4.27±0.02 | (dT-dA)7 | 0.1±0.1 | 9.0±0.6 | -4.7±0.6 |
| (rU-dA)8 | 228 ± 7 | 4.70±0.02 | (dT-dA)8 | 0.3±0.1 | 8.4±0.2 | -3.7±0.2 |
| (rU-dA)9 | 228 ± 5 | 4.70±0.01 | (dT-dA)9 | 1.0±0.2 | 7.7±0.1 | -3.0±0.1 |
| (rA-dT)10 | b | b | (dA-dT)10 | 1.4±0.2 | 7.6±0.1 | |
| (rU-dA)11 | 9.8 ± 0.4 | 6.47±0.02 | (dT-dA)11 | 1.9±0.3 | 7.4±0.1 | -0.9±0.1 |
a
in kcal/mol at 10°C
b
Imino proton resonance is not resolved in the NMR spectrum